RuvB-like 1

RuvB-like 1 (E. coli)

PDB rendering based on 2c9o.

Available structures
PDB	2c9o

Identifiers

Symbols

RUVBL1; ECP54; INO80H; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A

External IDs

OMIM: 603449 MGI: 1928760 HomoloGene: 37839 GeneCards: RUVBL1 Gene

EC number

3.6.4.12

Gene Ontology
Molecular function	• nucleotide binding • DNA helicase activity • protein binding • ATP binding • hydrolase activity • ATPase activity
Cellular component	• nucleus • nucleoplasm • nucleolus • cytoplasm • Golgi apparatus • microtubule organizing center • cytoskeleton • membrane • nuclear matrix • Ino80 complex • NuA4 histone acetyltransferase complex • intracellular membrane-bounded organelle • MLL1 complex
Biological process	• DNA repair • DNA recombination • nucleosome assembly • regulation of transcription from RNA polymerase II promoter • cell cycle • mitosis • spermatogenesis • chromatin modification • CenH3-containing nucleosome assembly at centromere • regulation of growth • histone H4 acetylation • histone H2A acetylation • cell division
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
127.78 – 127.87 Mb

Chr 6:
88.42 – 88.45 Mb

PubMed search

[1]

[2]

RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene.^[1] RUVBL1 can form a hexamer. The hexamer can form a dodecamer with RUVBL2 protein.^[2] Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.

Interactions

RuvB-like 1 has been shown to interact with RUVBL2,^[3] Beta-catenin,^[4] ACTL6A,^[5] Myc^[5]^[6] and EP400.^[6]

References

^ "Entrez Gene: RUVBL1 RuvB-like 1 (E. coli)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8607.
^ Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. doi:10.1016/j.jmb.2006.11.030. PMID 17157868.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
^ Bauer, A; Huber O, Kemler R (Dec. 1998). "Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 95 (25): 14787–92. doi:10.1073/pnas.95.25.14787. ISSN 0027-8424. PMC 24527. PMID 9843967. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=24527.
^ ^a ^b Park, Jeonghyeon; Wood Marcelo A, Cole Michael D (Mar. 2002). "BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation". Mol. Cell. Biol. (United States) 22 (5): 1307–16. doi:10.1128/MCB.22.5.1307-1316.2002. ISSN 0270-7306. PMC 134713. PMID 11839798. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134713.
^ ^a ^b Fuchs, M; Gerber J, Drapkin R, Sif S, Ikura T, Ogryzko V, Lane W S, Nakatani Y, Livingston D M (Aug. 2001). "The p400 complex is an essential E1A transformation target". Cell (United States) 106 (3): 297–307. doi:10.1016/S0092-8674(01)00450-0. ISSN 0092-8674. PMID 11509179.

Doyon Y, Côté J (2004). "The highly conserved and multifunctional NuA4 HAT complex.". Curr. Opin. Genet. Dev. 14 (2): 147–54. doi:10.1016/j.gde.2004.02.009. PMID 15196461.
Dawson SJ, White LA (1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin.". J. Infect. 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Kanemaki M, Makino Y, Yoshida T, et al. (1997). "Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB". Biochem. Biophys. Res. Commun. 235 (1): 64–8. doi:10.1006/bbrc.1997.6729. PMID 9196036.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Makino Y, Mimori T, Koike C, et al. (1998). "TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human". Biochem. Biophys. Res. Commun. 245 (3): 819–23. doi:10.1006/bbrc.1998.8504. PMID 9588198.
Qiu XB, Lin YL, Thome KC, et al. (1998). "An eukaryotic RuvB-like protein (RUVBL1) essential for growth". J. Biol. Chem. 273 (43): 27786–93. doi:10.1074/jbc.273.43.27786. PMID 9774387.
Holzmann K, Gerner C, Korosec T, et al. (1998). "Identification and characterization of the ubiquitously occurring nuclear matrix protein NMP 238". Biochem. Biophys. Res. Commun. 252 (1): 39–45. doi:10.1006/bbrc.1998.9604. PMID 9813143.
Bauer A, Huber O, Kemler R (1999). "Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein". Proc. Natl. Acad. Sci. U.S.A. 95 (25): 14787–92. doi:10.1073/pnas.95.25.14787. PMC 24527. PMID 9843967. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=24527.
Kanemaki M, Kurokawa Y, Matsu-ura T, et al. (1999). "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a". J. Biol. Chem. 274 (32): 22437–44. doi:10.1074/jbc.274.32.22437. PMID 10428817.
Salzer U, Kubicek M, Prohaska R (1999). "Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins". Biochim. Biophys. Acta 1446 (3): 365–70. PMID 10524211.
Wood MA, McMahon SB, Cole MD (2000). "An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc". Mol. Cell 5 (2): 321–30. doi:10.1016/S1097-2765(00)80427-X. PMID 10882073.
Makino Y, Kanemaki M, Koga A, et al. (2000). "Chromosome mapping and expression of human tip49 family genes". DNA Seq. 11 (1–2): 145–8. PMID 10902922.
Ikura T, Ogryzko VV, Grigoriev M, et al. (2000). "Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis". Cell 102 (4): 463–73. doi:10.1016/S0092-8674(00)00051-9. PMID 10966108.
Hawley SB, Tamura T, Miles LA (2001). "Purification, cloning, and characterization of a profibrinolytic plasminogen-binding protein, TIP49a". J. Biol. Chem. 276 (1): 179–86. doi:10.1074/jbc.M004919200. PMID 11027681.
Bauer A, Chauvet S, Huber O, et al. (2001). "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity". EMBO J. 19 (22): 6121–30. doi:10.1093/emboj/19.22.6121. PMC 305835. PMID 11080158. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305835.
Park J, Wood MA, Cole MD (2002). "BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation". Mol. Cell. Biol. 22 (5): 1307–16. doi:10.1128/MCB.22.5.1307-1316.2002. PMC 134713. PMID 11839798. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134713.
Watkins NJ, Dickmanns A, Lührmann R (2003). "Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP". Mol. Cell. Biol. 22 (23): 8342–52. doi:10.1128/MCB.22.23.8342-8352.2002. PMC 134055. PMID 12417735. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134055.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Cai Y, Jin J, Tomomori-Sato C, et al. (2003). "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex". J. Biol. Chem. 278 (44): 42733–6. doi:10.1074/jbc.C300389200. PMID 12963728.

PDB gallery

2c9o: 3D STRUCTURE OF THE HUMAN RUVB-LIKE HELICASE RUVBL1

RuvB-like 1

Interactions

References

Further reading